placeholder for plasmid library containing pegRNAs for 8mer insertions at PPIF promoter. pegRNAs were designed to introduce 8-bp sequence insertions at a fixed site -53 bp from the PPIF promoter transcription start site
Homo sapiens (male) Jurkat cell line treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Homo sapiens (male) Jurkat cell line treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library