{
"@context": "/terms/",
"@id": "/in-vitro-systems/IGVFSM4726LPSQ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4726LPSQ",
"age": "unknown",
"aliases": [
"lea-starita:F9-DMS-Heavy-chain-antibody-Tile2-Replicate1-Bin3_idan"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM4726LPSQ](/in-vitro-systems/IGVFSM4726LPSQ/) has in progress subobject construct library set [IGVFDS1009OBGA](/construct-library-sets/IGVFDS1009OBGA/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM4726LPSQ/"
}
],
"NOT_COMPLIANT": [
{
"category": "missing plasmid map",
"detail": "Construct library set [IGVFDS1009OBGA](/construct-library-sets/IGVFDS1009OBGA/) does not have a plasmid map attached in `documents`. Construct library sets are expected to be associated with a plasmid map document.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_construct_library_set_plasmid_map",
"path": "/construct-library-sets/IGVFDS1009OBGA/"
}
],
"WARNING": [
{
"category": "missing files",
"detail": "Construct library set [IGVFDS1009OBGA](/construct-library-sets/IGVFDS1009OBGA/) has no `files`. File sets are expected to have files.",
"level": 40,
"level_name": "WARNING",
"name": "audit_no_files",
"path": "/construct-library-sets/IGVFDS1009OBGA/"
}
]
},
"award": {
"@id": "/awards/HG011969/",
"component": "functional characterization"
},
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS1009OBGA/",
"accession": "IGVFDS1009OBGA",
"file_set_type": "expression vector library"
}
],
"creation_timestamp": "2024-03-29T00:19:47.896468+00:00",
"demultiplexed_to": [],
"description": "poly-G384A cells containing F9 deep mutational scanning library incubated with Heavy chain antibody. Sequencing tile 2, biological replicate 1, bin 3.",
"donors": [
"/human-donors/IGVFDO1183RRBM/"
],
"file_sets": [
{
"@id": "/measurement-sets/IGVFDS1953KIOT/",
"accession": "IGVFDS1953KIOT",
"aliases": [
"lea-starita:F9-DMS-Heavy-chain-antibody-Tile2-Replicate1-Bin3-FileSet_idan",
"igvf:my-alias"
],
"file_set_type": "experimental data",
"lab": {
"title": "Doug Fowler, UW"
},
"preferred_assay_title": "VAMP-seq",
"status": "released",
"summary": "VAMP-seq integrating a expression vector library of tile tile3 of INTS11 (AA 299-461) (sequence variants)"
},
{
"@id": "/analysis-sets/IGVFDS7736VVSH/",
"accession": "IGVFDS7736VVSH",
"aliases": [
"lea-starita:F9-DMS-Scores_idan"
],
"file_set_type": "principal analysis",
"lab": {
"title": "Doug Fowler, UW"
},
"status": "in progress",
"summary": "VAMP-seq"
}
],
"institutional_certificates": [],
"lab": {
"@id": "/labs/doug-fowler/",
"title": "Doug Fowler, UW"
},
"moi": 1,
"multiplexed_in": [],
"notes": "The \"classification\" of this in vitro system was moved into the array property \"classifications.\"",
"nucleic_acid_delivery": "transfection",
"origin_of": [],
"parts": [],
"pooled_in": [],
"release_timestamp": "2024-03-22T23:04:37.145369+00:00",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001184/",
"term_name": "HEK293T"
}
],
"schema_version": "23",
"sex": "female",
"sorted_fractions": [],
"sources": [
{
"@id": "/labs/doug-fowler/",
"title": "Doug Fowler, UW"
}
],
"status": "released",
"submitted_by": {
"@id": "/users/98fb23d3-0d79-4c3d-981d-01539e6589f1/",
"title": "Idan Gabdank"
},
"summary": "HEK293T cell line, female, Homo sapiens transfected with a expression vector library (MOI of 1)",
"taxa": "Homo sapiens",
"uuid": "103c6e2d-7d50-49bc-9e8d-a6f3cc0fc93d",
"virtual": false
}