Homo sapiens (female) HEK293T cell line transfected with a expression vector library (MOI of 1), Homo sapiens (male) THP-1 cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Homo sapiens HEK293T, THP-1 cell line, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library, expression vector library
CRISPR prime editing VAMP-seq integrating an expression vector library of tile tile3 of INTS11 (AA 299-461) (sequence variants) and a guide (sgRNA) library targeting sequence variants in a genomic locus