in vitro CRISPR screen assay

VAMP-seq

Homo sapiens (male) THP-1 cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

CRISPR prime editing VAMP-seq integrating a guide (sgRNA) library targeting sequence variants in a genomic locus

in vitro CRISPR screen using flow cytometry

Variant-EFFECTS

Homo sapiens (male) Jurkat cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus

single-cell ATAC-seq

reads, seqspec, PacBio subreads

fastq, yaml, bam

VAMP-seq

Homo sapiens (female) K562 cell line transfected with a guide library

CRISPR VAMP-seq integrating a guide (sgRNA) library targeting TF binding sites, phenotype-associated variants genome-wide associated with DiGeorge syndrome

single-cell RNA sequencing assay

scRNA-seq

multiplexed sample: Homo sapiens (female) H9 differentiated cell specimen induced to neural progenitor cell for 30 minutes (cellular sub pool: K80_A2); Homo sapiens (male) endothelial cell line (sorting details: detail of the fraction) depleted of lipopolysaccharide for 6 hours, transfected with a guide library; ... and 4 more samples

CRISPR scRNA-seq integrating a guide (sgRNA) library targeting TF binding sites, phenotype-associated variants genome-wide associated with DiGeorge syndrome

SHARE-seq

raw signal, signal p-value

bigWig

SHARE-seq

Mus musculus B6XCast (male, 15-25 months) adrenal gland tissue (cellular sub pool: AT9029) associated with pulmonary arterial hypertension, treated with 10 mM leucine for 6 hours

SHARE-seq (SHARE-seq)